Strain Data Sheet

RBRC05385

Strain Information

Image
BRC No.RBRC05385
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Dppa3-cre/Esr1*)9Sait/SaitRbrc
Former Common nameDppa3-MER-Cre-MER-PEST (Acc. No. CDB0475T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takayuki Hirota and Mitinori Saitou, Graduate School of Medicine, Kyoto University in 2011. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThis transgenic mouse expresses MER-Cre-MER-PEST protein under the control of the regulatory elements of the Dppa3. This strain enables 4-Hydroxytamoxifen-dependent Cre recombination in preimplantation embryos, primordial germ cells, and oocytes.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
References
Drug-inducible gene recombination by the Dppa3-MER Cre MER transgene in the developmental cycle of the germ cell lineage in mice.
Hirota T, Ohta H, Shigeta M, Niwa H, Saitou M
Biol. Reprod., 85, 367-377 (2011). 21525417

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyAUN
Esr1*estrogen receptor 1 (alpha), mouse ER with a mutation G525R)UNEsr1*
FLAGFLAG tag (synthetic)UNFLAG
Odc1mouse ornithine decarboxylase (PEST sequence) cDNAUNOdc1
crePhage P1 Cre recombinaseUNcre
hyghygromycin phasphotransferase (E. coli)UNhyg
neoneomycin resistance gene (E. coli)UN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Dppa3 promoter, FLAG epitope tag (synthetic), mouse mutated estrogen receptor (Mer) cDNA, Phage P1 Cre recombinase, mouse PEST sequence (proline, glutamic acid, serine, and threonine) of ornithine decarboxylase (residues 1402-1524), SV40 polyA, E. coli hygromycin resistance gene, E. coli neo, Phage P1 loxP derived from pBACe3.6
    Research applicationCre/loxP system
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0475T and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Biol Reprod. 2011 85(2):367-77.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2013

    BRC mice in Publications

    Yohei Hayashi, Jintaro Kaneko, Yumi Ito-Matsuoka, Asuka Takehara, Mayuka Funakoshi, So Maezawa, Kenjiro Shirane, Shigeki Furuya, Yasuhisa Matsui
    Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolism
    iScience 110702(2024)