Strain Data Sheet

RBRC05385

Information1

Image
BRC No.RBRC05385
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Dppa3-cre/Esr1*)9Sait/SaitRbrc
Former Common nameDppa3-MER-Cre-MER-PEST (Acc. No. CDB0475T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takayuki Hirota and Mitinori Saitou, Graduate School of Medicine, Kyoto University in 2011. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThis transgenic mouse expresses MER-Cre-MER-PEST protein under the control of the regulatory elements of the Dppa3. This strain enables 4-Hydroxytamoxifen-dependent Cre recombination in preimplantation embryos, primordial germ cells, and oocytes.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
ReferencesBiol. Reprod., 85, 367-377 (2011). 21525417

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Esr1*estrogen receptor 1 (alpha), mouse ER with a mutation G525R)UNEsr1*

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Odc1mouse ornithine decarboxylase (PEST sequence) cDNAUNOdc1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinaseUNcre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
hyghygromycin phasphotransferase (E. coli)UNhyg

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse Dppa3 promoter, FLAG epitope tag (synthetic), mouse mutated estrogen receptor (Mer) cDNA, Phage P1 Cre recombinase, mouse PEST sequence (proline, glutamic acid, serine, and threonine) of ornithine decarboxylase (residues 1402-1524), SV40 polyA, E. coli hygromycin resistance gene, E. coli neo, Phage P1 loxP derived from pBACe3.6
Research applicationCre/loxP system
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0475T and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Biol Reprod. 2011 85(2):367-77.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Aug 2013
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data