RBRC05384

Information1

Image
BRC No.RBRC05384
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prdm14-Venus)1Sait/SaitRbrc
Former Common namePrdm14-mVenus (Acc. No. CDB0461T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThis transgenic strain expresses a plasma membrane anchored Venus (mVenus) under the control of the mouse Prdm14 promoter.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
ReferencesNat. Genet., 40, 1016-1022 (2008). 18622394

Health Report

Examination Date / Room / Rack2021/02/15Room:3-CRack:I
2020/11/16Room:3-CRack:I
2020/08/24Room:3-CRack:I
2020/06/01Room:3-CRack:I
2020/02/17Room:3-CRack:I
2014/02/18Room:3-CRack:I
2013/12/16Room:3-CRack:I
2013/10/30Room:3-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)UNGH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prdm14PR domain containing 14UNPrdm14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(Prdm14-Venus)1SaitUNTg(Prdm14-Venus)1Saittransgene insertion 1, Mitinori Saitou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mVenusyellow fluorescent protein (Aequorea victoria) A206KUNmVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse Igk leader sequenceUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PDGF receptor anchoring domainUN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
chimeric intron of the pCL neo vectorUN

Information2

Donor DNAchimeric intron of the pCL neo vector, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus), mouse Igk leader sequence, PDGF receptor anchoring domain, Bovine growth hormone polyadenylation signal sequence, mouse Prdm14 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0461T and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat Genet. 2008 40(8):1016-22.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Mouse of the Month Aug 2013
Genotyping protocol -PCR-
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).

BRC mice in Publications

No Data