Strain Data Sheet

RBRC05384

Strain Information

Image
BRC No.RBRC05384
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prdm14-Venus)1Sait/SaitRbrc
Former Common namePrdm14-mVenus (Acc. No. CDB0461T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThis transgenic strain expresses a plasma membrane anchored Venus (mVenus) under the control of the mouse Prdm14 promoter.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
References
Critical function of Prdm14 for the establishment of the germ cell lineage in mice.
Yamaji M, Seki Y, Kurimoto K, Yabuta Y, Yuasa M, Shigeta M, Yamanaka K, Ohinata Y, Saitou M
Nat. Genet., 40, 1016-1022 (2008). 18622394

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GH Growth hormone polyA (Bovine)UNGH
Prdm14 PR domain containing 14UNPrdm14
Tg(Prdm14-Venus)1Sait UNTg(Prdm14-Venus)1Sait
MGI:3805974		transgene insertion 1, Mitinori Saitou
mVenus Yellow Fluorescent Protein (Aequorea victoria)UNmVenus
chimeric intron of the pCL neo vectorUN
PDGF receptor anchoring domainUN
mouse Igk leader sequenceUN

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased primordial germ cell number(MP:0008392)
    • Detailed phenotype data

    Ordering Information

    Donor DNAchimeric intron of the pCL neo vector, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus), mouse Igk leader sequence, PDGF receptor anchoring domain, Bovine growth hormone polyadenylation signal sequence, mouse Prdm14 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0461T and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat Genet. 2008 40(8):1016-22.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. (5) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2013

    BRC mice in Publications

    Grosswendt S, Kretzmer H, Smith ZD, Kumar AS, Hetzel S, Wittler L, Klages S, Timmermann B, Mukherji S, Meissner A.
    Epigenetic regulator function through mouse gastrulation.
    Nature 584(7819) 102-108(2020) 32728215