Strain Data Sheet

RBRC05383

Information1

Image
BRC No.RBRC05383
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prdm1-Venus)#Sait/Rbrc
Former Common nameBrimp1-mVenus transgenic mouse, Blimp1-mVenus (Acc. No. CDB0460T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThe transgenic mice having a Prdm1-mVenus containing a Venus reporter sequence with an Igk leader sequence, platelet derived growth factor transmembrane sequence and bovine growth hormone polyA signal that inserted in-frame after the initial ATG exon 3 of Pdrm1 BAC gene. Tg homozygous mice are viable and fertile.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
ReferencesReproduction, 136, 503-514 (2008). 18583473
Nature. 501(7466):222-226 (2013). 23913270

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)2GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence2HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tg(Prdm1-Venus)#Sait2Tg(Prdm1-Venus)#Saittransgene insertion, Mitinori Saitou

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mVenusYellow Fluorescent Protein (Aequorea victoria)2mVenusmouse Blimp1 promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
myc-tagmyc-epitope tag2myc-tag

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PDGF receptor anchoring domain2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse Igk leader sequence2

Information2

Donor DNAmouse Blimp1 genomic DNA, mouse Igk leader sequence, influenza virus hemagglutinin (HA) tag, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus) cDNA, c-Myc epitope tag, mouse PDGF receptor anchoring domain cDNA, Stop, Bovine growth hormone (BGH) polyadenylation signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0460T and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Reproduction. 2008 136(4):503-14.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Aug 2013
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data