Strain Data Sheet

RBRC05383

Strain Information

Image
BRC No.RBRC05383
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Prdm1-Venus)#Sait/Rbrc
Former Common nameBrimp1-mVenus transgenic mouse, Blimp1-mVenus (Acc. No. CDB0460T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThe transgenic mice having a Prdm1-mVenus containing a Venus reporter sequence with an Igk leader sequence, platelet derived growth factor transmembrane sequence and bovine growth hormone polyA signal that inserted in-frame after the initial ATG exon 3 of Pdrm1 BAC gene. Tg homozygous mice are viable and fertile.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
References
A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter.
Ohinata Y, Sano M, Shigeta M, Yamanaka K, Saitou M
Reproduction, 136, 503-514 (2008). 18583473

Induction of mouse germ-cell fate by transcription factors in vitro.
Nakaki F, Hayashi K, Ohta H, Kurimoto K, Yabuta Y, Saitou M
Nature. 501(7466):222-226 (2013). 23913270

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
PDGF receptor anchoring domain2
mouse Igk leader sequence2
GHGrowth hormone polyA (Bovine)2GH
HAhuman influenza virus HA (hemagglutinin) tag sequence2HA
Tg(Prdm1-Venus)#Sait2Tg(Prdm1-Venus)#Saittransgene insertion, Mitinori Saitou
mVenusYellow Fluorescent Protein (Aequorea victoria)2mVenusmouse Blimp1 promoter
myc-tagmyc-epitope tag2myc-tag

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Blimp1 genomic DNA, mouse Igk leader sequence, influenza virus hemagglutinin (HA) tag, Jellyfish Variant of enhanced yellow fluorescent protein (mVenus) cDNA, c-Myc epitope tag, mouse PDGF receptor anchoring domain cDNA, Stop, Bovine growth hormone (BGH) polyadenylation signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0460T and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Reproduction. 2008 136(4):503-14.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. (5) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Aug 2013

    BRC mice in Publications

    No Data