RBRC05382

Information1

Image
BRC No.RBRC05382
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Dppa3-ECFP)#Sait/Rbrc
Former Common nameStella-ECFP (Acc. No. CDB0465T)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yasuhide Ohinata and Mitinori Saitou, RIKEN, Center for Developmental Biology in 2008. Transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were crossed to C57BL/6.
Strain descriptionThe transgenic mice having a Dppa3-ECFP containing upstream sequence, exon 1 through exon 4 of Dppa3 with a ECFP reporter sequence that inserted in-frame after the initial ATG of exon 2 of the Dppa3. Tg homozygous females have low fertility.
Colony maintenanceCarrier x Nocarrier [C57BL/6N]
ReferencesReproduction., 136, 503-514 (2008). 18583473
Nature. (2013). 23913270

Health Report

Examination Date / Room / Rack2015/04/20Room:3-CRack:D
2015/02/16Room:3-CRack:D
2014/12/15Room:3-CRack:D
2014/10/20Room:3-CRack:D
2014/08/18Room:3-CRack:D
2014/06/16Room:3-CRack:D
2014/04/21Room:3-CRack:D
2014/02/18Room:3-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
ECFPEnhanced Cyan Fluorescent Protein (Aequorea victoria), F64L/S65T/K212N/H231L1ECFPtransgene insertion, Mitinori Saitoumouse Dppa3 promoter

Information2

Donor DNAmouse Dppa3 genomic DNA, Jellyfish ECFP (GFP variant) cDNA, SV40 polyA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitinori Saitou, Kyoto University, E-mail: saitou@anat2.med.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0465T and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Reproduction. 2008 136(4):503-14.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Mouse of the Month Aug 2013

BRC mice in Publications

No Data