RBRC05363

Information1

Image
BRC No.RBRC05363
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameSTOCK Tg(FLEXCAG-VSFP2.3)511Bsi
Former Common nameCAG-VSFP2.3/floxed Tg#511
H-2 Haplotype
ES Cell line
Background strainJcl:B6D2F1
Appearance
Strain developmentDeveloped by Thomas Knoepfel, RIKEN Brain Science Institute. Developed by Thomas Knoepfel, RIKEN Brain Science Institute. C57BL/6, DBA/2 and ICR mixed.
Strain descriptionMice carry the gene for the voltage sensor VSFP2.3 flanked by two sets of loxP sites; expression will be activated by Cre recombinase following crossing with a Cre driver mouse. Line 511 (RBRC05363), Line 538 (RBRC05364), Line 555 (RBRC05365) and Line 564 (RBRC05366). Expression levels of these strains are under analysis.
Colony maintenanceCarrier x Noncarrier [or Crossing to Jcl:B6D2F1]
References

Health Report

Examination Date / Room / Rack2014/02/18Room:3-ARack:K
2013/12/16Room:3-ARack:K
2013/10/21Room:3-ARack:K
2013/08/26Room:3-ARack:K
2013/06/17Room:3-ARack:K
2013/04/15Room:3-ARack:K
2013/02/18Room:3-ARack:K
2012/12/10Room:3-ARack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
VSFP2.3voltage-sensitive fluorescent protein 2.3UNVSFP2.3CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory elementUNWPRE

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyAUN

Information2

Donor DNAThe name of the nucleic acid, or replicated product Ci-VSP / Ciona intestinalis, EYFP and CFP / Aequoria victoria, poly-A / SV40, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Cytomegalovirus enhancer / cytomegalovirus fragment, chicken / beta action promoter, rabbit / beta-globulin 3 prime flanking sequences
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorThomas Knöepfel (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Contact Information

BRC mice in Publications

No Data