Strain Data Sheet

RBRC05324

Strain Information

Image
BRC No.RBRC05324
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Sirt1<tm2.1Mcby>
Former Common nameSirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A knock-in vector carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine) was inserted into exon 5. A hygromycin resistant cassette flanked by loxP sites was removed by crossing with Prm-Cre mice (129-Tg (Prm-Cre) 580g / J).
Strain descriptionSirT1 variant (H355Y) knock-in mice, carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine). Homozygous mutant mice are infertile.
Colony maintenanceHeterozygote X Wild-type
References
SirT1 catalytic activity is required for male fertility and metabolic homeostasis in mice.
Seifert E L, Caron A Z, Morin K, Coulombe J, He X H, Jardine K, Dewar-Darch D, Boekelheide K, Harper M E, McBurney M W
FASEB J., 26(2):555-66 (2011). 22006156

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sirt1sirtuin 110Sirt1targeted mutation 2.1, Michael W McBurney
loxPphage P1 loxP10loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal craniofacial morphology(MP:0000428)

  • abnormal energy expenditure(MP:0005450)

  • abnormal eyelid morphology(MP:0001340)

  • abnormal glucose homeostasis(MP:0002078)

  • abnormal glucose tolerance(MP:0005291)
    • more 20 phenotypes
  • abnormal lacrimal gland morphology(MP:0001346)

  • abnormal salivary gland morphology(MP:0000613)

  • abnormal spermatogenesis(MP:0001156)

  • asthenozoospermia(MP:0002675)

  • decreased body size(MP:0001265)

  • decreased circulating insulin level(MP:0002727)

  • decreased respiratory quotient(MP:0010379)

  • decreased white adipose tissue amount(MP:0001783)

  • enlarged heart(MP:0000274)

  • enlarged pancreas(MP:0003450)

  • enlarged spleen(MP:0000691)

  • hyperpnea(MP:0010946)

  • increased brain size(MP:0005238)

  • increased food intake(MP:0011939)

  • increased oxygen consumption(MP:0005289)

  • male infertility(MP:0001925)

  • oligozoospermia(MP:0002687)

  • perinatal lethality(MP:0002081)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • short snout(MP:0000445)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, mouse Sirt1 genomic DNA
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.　　The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorMichael W. McBurney (Ottawa Hospital Research Institute)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data