BRC No.RBRC05324
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Sirt1<tm2.1Mcby>
Former Common nameSirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Strain developmentDeveloped by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A knock-in vector carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine) was inserted into exon 5. A hygromycin resistant cassette flanked by loxP sites was removed by crossing with Prm-Cre mice (129-Tg (Prm-Cre) 580g / J).
Strain descriptionSirT1 variant (H355Y) knock-in mice, carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine). Homozygous mutant mice are infertile.
Colony maintenanceHeterozygote X Wild-type
ReferencesFASEB J., 26(2):555-66 (2011). 22006156

Health Report

Examination Date / Room / Rack2019/09/30Room:4-CRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sirt1sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae)10Sirt1<tm2.1Mcby>targeted mutation 2.1, Michael W McBurney

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP10loxP


Donor DNAPhage P1 loxP site, mouse Sirt1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMichael W. McBurney (Ottawa Hospital Research Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data