RBRC05323

Information1

Image
BRC No.RBRC05323
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Sirt1<tm1Mcby>
Former Common nameSTOCK Sirt1<tm1Mcby>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A hygromycin resistance cassette was inserted to replace exons 5 and 6. 129 background.
Strain descriptionSirT1 knockout mice. A hygromycin resistance cassette was inserted to replace exons 5 and 6 of Sirt1 gene. Homozygous mutant mice die at birth. When outbred to CD1, SirT1 KO mice have short snouts, eyelid defect and are smaller than littermates at weaning.
Colony maintenanceHeterozygote X Wild-type
ReferencesMol. Cell. Biol., 23, 38-54 (2003). 12482959

Health Report

Examination Date / Room / Rack2018/10/15Room:3-CRack:E
2018/08/20Room:3-CRack:E
2018/06/18Room:3-CRack:E
2018/04/16Room:3-CRack:E
2018/02/19Room:3-CRack:E
2017/12/18Room:3-CRack:E
2017/10/16Room:3-CRack:E
2017/08/21Room:3-CRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sirt1sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae)10Sirt1<tm1Mcby>targeted mutation 1, Michael W McBurney

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Hyghygromycin phasphotransferase (E. coli)10Hygherpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)10

Information2

Donor DNAmouse Pgk1 splice acceptor (SA), Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Hygromycin resistance gene, mouse Pgk1 splice donor (SD), mouse Sirt1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMichael W. McBurney (Ottawa Hospital Research Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

Kumar A, Daitsh Y, Ben-Aderet L, Qiq O, Elayyan J, Batshon G, Reich E, Maatuf YH, Engel S, Dvir-Ginzberg M.
A predicted unstructured C-terminal loop domain in SIRT1 is required for cathepsin B cleavage.
J. Cell. Sci. (2018) 30054388

Tomita T, Hamazaki J, Hirayama S, McBurney MW, Yashiroda H, Murata S.
Sirt1-deficiency causes defective protein quality control.
Sci Rep (2015) 26219988