Strain Data Sheet

RBRC05290

Information1

Image
BRC No.RBRC05290
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dscam<tm1.1Kzy>/A151Kzy
Former Common nameB6-Dscam KO#A151
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentExon 1 was removed by crossing with CAG-cre (B6 background). The mice were backcrossed to C57BL/6 over 21 times.
Strain descriptionDscam knockout mice. Dscam, Down syndrome cell adhesion molecule is a gene of neural Ig superfamily mediates cell adhesion and axon outgrowth. Homozygous mutant mice of C57BL/6 genetic background die within 24 hours after birth due to central respiratory defects. In BALB/c mixed background, Homozygous mutant mice are viable and survive to adulthood.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesJ. Neurosci., 29, 2984-2996 (2009). 19261893

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DscamDown syndrome cell adhesion molecule16Dscam<tm1.1Kzy>targeted mutation 1.1, Kazuhiro Yamakawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP16loxP

Information2

Donor DNAP1 Phage loxP site, mouse Dscam genomic DNA
Research applicationCre/loxP system
Mouse Models for Human Disease
Neurobiology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Amano et al., J. Neurosci., 29(9):2984-2996. 2009In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuhiro Yamakawa (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Jan 2012
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

Lim CS, Kim MJ, Choi JE, Islam MA, Lee YK, Xiong Y, Shim KW, Yang JE, Lee RU, Lee J, Park P, Kwak JH, Seo H, Kim CH, Lee JH, Lee YS, Hwang SK, Lee K, Lee JA, Kaang BK.
Dysfunction of NMDA receptors in neuronal models of an autism spectrum disorder patient with a DSCAM mutation and in Dscam-knockout mice.
Mol Psychiatry (2021) 34253863