RBRC05289

Information1

Image
BRC No.RBRC05289
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameD2;B6-Tg(CAG-EGFP)C14-Y01-FM131Osb
Former Common nameD2-CAG-GFP#131
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Kei Ishii, Graduate School of Medicine, Kyoto University. C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb mice were backcrossed to DBA/2 (N5, Oct 2011)
Strain descriptionThis transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. DBA/2 background.
Colony maintenanceCarrier x Noncarrier [or Crossing to DBA/2CrSlc]
ReferencesBiol. Reprod., 69(2):612-616 (2003). 12700182
Stem Cell Rep., (2015). 26095606

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)14transgene insertion 131, Research Institute for Microbial Diseases, Osaka UniversityCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Jellyfish GFP cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 69(2):612-616 (2003). Stem Cell Rep., (2015).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must make MTA specified for Green Mouse with Prof. Masaru Okabe, Osaka University prior to obtaining the BIOLOGICAL RESOURCE.
DepositorTakashi Shinohara (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data