Strain Data Sheet

RBRC05286

Information1

Image
BRC No.RBRC05286
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mdga1<tm1.1Tymo>/TymoRbrc
Former Common nameMdga1 KO, B6-Mdga1 KO, Mdga1 (Acc. No.CDB0454K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionMdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of meurons during corticogenesis.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesAm. J. Med. Genet. B Neuropsychiatr. Genet., 147B, 1089-1100 (2008). 18384059
Schizophr. Res., 194-200 (2011). 21146959
Dev. Dyn., 240, 96-107 (2011). 21104742
Brain Res., 1101, 12-19 (2006). 16782075

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mdga1MAM domain containing glycosylphosphatidylinositol anchor 117Mdga1 <tm1.1Tymo>targeted mutation 1.1, Toru Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence17lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAE.coli LacZ, P1 phage lox71, loxP, Yeast frt, マウス PGKプロモーター,PGK 3’end, ウサギ beta-globin 3’end
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Tohru Yamamoto, Hokkaido University, E-mail: yamamoto@pharm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Dyn. 2011 240(1):96-107.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data