Strain Data Sheet

RBRC05286

Strain Information

Image
BRC No.RBRC05286
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mdga1<tm1.1Tymo>/TymoRbrc
Former Common nameMdga1 KO, B6-Mdga1 KO, Mdga1 (Acc. No.CDB0454K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionMdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of meurons during corticogenesis.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
MDGA1, an IgSF molecule containing a MAM domain, heterophilically associates with axon- and muscle-associated binding partners through distinct structural domains.
Yuiko Fujimura, Misato Iwashita, Fumio Matsuzaki, Tohru Yamamoto
Brain Res., 1101(1):12-9 (2006). 16782075

Association analysis of schizophrenia on 18 genes involved in neuronal migration: MDGA1 as a new susceptibility gene.
Anna K Kähler, Srdjan Djurovic, Bettina Kulle, Erik G Jönsson, Ingrid Agartz, Håkan Hall, Stein Opjordsmoen, Klaus D Jakobsen, Thomas Hansen, Ingrid Melle, Thomas Werge, Vidar M Steen, Ole A Andreassen
Am. J. Med. Genet. B Neuropsychiatr. Genet., 147B(7):1089-100 (2008). 18384059

IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons.
Takao Ishikawa, Naoya Gotoh, Chiaki Murayama, Takaya Abe, Misato Iwashita, Fumio Matsuzaki, Toshiharu Suzuki, Tohru Yamamoto
Dev. Dyn., 240(1):96-107 (2011). 21104742

The MDGA1 gene confers risk to schizophrenia and bipolar disorder.
Junyan Li, Jie Liu, Guoyin Feng, Tao Li, Qian Zhao, You Li, Zhiwei Hu, Linqing Zheng, Zhen Zeng, Lin He, Ti Wang, Yongyong Shi
Schizophr. Res., 125(2-3):194-200 (2011). 21146959

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site17Frt
Mdga1
MGI:1922012		MAM domain containing glycosylphosphatidylinositol anchor 117Mdga1 <tm1.1Tymo>
MGI:4878863		targeted mutation 1.1, Toru Yamamoto
lacZ beta-galactosidase (E. coli)17
lox71 mutant loxP17lox71
loxP phage P1 loxP17loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal neuronal migration (MP:0006009)

  • abnormal prepulse inhibition (MP:0003088)

  • decreased prepulse inhibition (MP:0009142)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE.coli LacZ, P1 phage lox71, loxP, Yeast frt, マウス PGKプロモーター,PGK 3’end, ウサギ beta-globin 3’end
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 240(1):96-107 (2011). The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Tohru Yamamoto, Hokkaido University, E-mail: yamamoto@pharm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data