Strain Information | |
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Image | |
BRC No. | RBRC05286 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6.Cg-Mdga1<tm1.1Tymo>/TymoRbrc |
Former Common name | Mdga1 KO, B6-Mdga1 KO, Mdga1 (Acc. No.CDB0454K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6J. |
Strain description | Mdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of meurons during corticogenesis. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | MDGA1, an IgSF molecule containing a MAM domain, heterophilically associates with axon- and muscle-associated binding partners through distinct structural domains. Fujimura Y, Iwashita M, Matsuzaki F, Yamamoto T Brain Res., 1101, 12-19 (2006). 16782075Association analysis of schizophrenia on 18 genes involved in neuronal migration: MDGA1 as a new susceptibility gene. Kahler A K, Djurovic S, Kulle B, Jonsson E G, Agartz I, Hall H, Opjordsmoen S, Jakobsen K D, Hansen T, Melle I, Werge T, Steen V M, Andreassen O A Am. J. Med. Genet. B Neuropsychiatr. Genet., 147B, 1089-1100 (2008). 18384059The MDGA1 gene confers risk to schizophrenia and bipolar disorder. Li J, Liu J, Feng G, Li T, Zhao Q, Li Y, Hu Z, Zheng L, Zeng Z, He L, Wang T, Shi Y Schizophr. Res., 194-200 (2011). 21146959IgSF molecule MDGA1 is involved in radial migration and positioning of a subset of cortical upper-layer neurons. Ishikawa T, Gotoh N, Murayama C, Abe T, Iwashita M, Matsuzaki F, Suzuki T, Yamamoto T Dev. Dyn., 240, 96-107 (2011). 21104742 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Frt | yeast FRT (flippase recombination target) site | 17 | Frt | ||||
Mdga1 MGI:1922012 | MAM domain containing glycosylphosphatidylinositol anchor 1 | 17 | Mdga1 <tm1.1Tymo> MGI:4878863 | targeted mutation 1.1, Toru Yamamoto | |||
lacZ | beta-galactosidase (E. coli) | 17 | |||||
lox71 | mutant lox sequence | 17 | lox71 | ||||
loxP | phage P1 loxP | 17 | loxP |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | E.coli LacZ, P1 phage lox71, loxP, Yeast frt, マウス PGKプロモーター,PGK 3’end, ウサギ beta-globin 3’end |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Tohru Yamamoto, Hokkaido University, E-mail: yamamoto@pharm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Dyn. 2011 240(1):96-107.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | ![]() ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |