Strain Data Sheet

RBRC05284

Strain Information

Image
BRC No.RBRC05284
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prom1<tm1Toko>/Rbrc
Former Common nameProminin1(floxed-lacZ-DTA), Prominin1 (Acc. No. CDB0623K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toru Kondo, RIKEN Center for Developmental Biology.
Strain descriptionProm1, prominin 1 knockout mice (Prominin1 (floxed-lacZ-DTA)). Exon 2, which contains the first ATG codon on the Prom1 locus was replaced with floxed-lacZ and DTA. Homozygous mice are viable and fertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
Glioblastoma formation from cell population depleted of Prominin1-expressing cells.
Nishide K, Nakatani Y, Kiyonari H, Kondo T
PLoS One, 4, e6869 (2009). 19718438

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
DTdiphtheria toxin A (DT-A)5DT
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • Frtyeast FRT (flippase recombination target) site5Frt
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • HBB2rabbit beta-globin polyA5HBB2
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • Prom1prominin 15Prom1 targeted mutation 1, Toru Kondo
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • lacZbeta-galactosidase (E. coli)5
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • loxPphage P1 loxP5loxP
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)
    • loxPphage P1 loxP5loxP
  • cone-rod dystrophy 12(MedGEN)

  • retinal macular dystrophy type 2(MedGEN)

  • retinitis pigmentosa 41(DisGeNET, MedGEN)
    • more 1 Diseases
  • Stargardt disease 4(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 LoxP, E. coli lacZ, rabbit beta globin pA, diphtheria toxin DT-A, pA, yeast FRT (flipase recombination target) site, mouse Prom1 genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Toru Kondo, RIKEN, E-mail: tkondo@igm.hokudai.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0623K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (PLoS One. 2009 4(8):e6869.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data