RBRC05283

Information1

Image
BRC No.RBRC05283
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameNoto(nmCherry-CreERT2) (Acc. No. CDB0579K)
Former Common nameNoto(nmCherry-CreERT2) (Acc. No. CDB0579K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Sasaki, RIKEN Center for Developmental Biology.
Strain descriptionmCherry-creER2 Noto knockin mice. About 50% of homozygotes display varying degrees of tail defects.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N] About 50% of homozygotes display varying degrees of tail defects.
ReferencesMech. Dev., 126, 791-803 (2009). 19720144

Health Report

Examination Date / Room / Rack2020/02/17Room:3-CRack:A
2019/12/16Room:3-CRack:A
2019/10/21Room:3-CRack:A
2019/08/26Room:3-CRack:A
2019/07/22Room:3-CRack:A
2019/06/17Room:3-CRack:A
2019/04/15Room:3-CRack:A
2019/02/18Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Notonotochord homolog (Xenopus laevis)6Noto<tm1(cre/ERT2)Hssk>targeted mutation 1, Hiroshi Sasaki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
E2AEquine rhinitis A virus (ERAV) 2A peptide6E2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)6ERT2

Gene symbolGene nameChr.Allele symbolAllele namePromoter
H2Bhuman Histone H2B cDNA6H2B

Gene symbolGene nameChr.Allele symbolAllele namePromoter
T2AThosea asigna virus 2A peptide (insect)6T2A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase6cre

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Information2

Donor DNAE2A [equine rhinitis A virus 2A peptide], human histone H2B cDNA,Discosoma sp. mCherry cDNA, T2A [Thosea asigna virus 2A peptide], Phage P1 Cre recombinase DNA, human Estrogen receptor cDNA, Phage P1 loxP site, mouse Noto genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0579K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mech Dev. 2009 126(10):791-803.) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.UCSD MTA
Genotyping protocol -PCR-

BRC mice in Publications

No Data