Strain Data Sheet

RBRC05283

Strain Information

Image
BRC No.RBRC05283
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Noto<tm1(cre/ERT2)Hssk>/Rbrc
Former Common nameNoto(nmCherry-CreERT2) (Acc. No. CDB0579K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Hiroshi Sasaki, RIKEN Center for Developmental Biology.
Strain descriptionmCherry-creER2 Noto knockin mice. About 50% of homozygotes display varying degrees of tail defects.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N] About 50% of homozygotes display varying degrees of tail defects.
References
Wnt signaling maintains the notochord fate for progenitor cells and supports the posterior extension of the notochord.
Ukita K, Hirahara S, Oshima N, Imuta Y, Yoshimoto A, Jang C W, Oginuma M, Saga Y, Behringer R R, Kondoh H, Sasaki H
Mech. Dev., 126, 791-803 (2009). 19720144

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
E2AEquine rhinitis A virus (ERAV) 2A peptide6E2A
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)6ERT2
H2Bhuman Histone H2B cDNA6H2B
Notonotochord homeobox6Nototargeted mutation 1, Hiroshi Sasaki
T2AThosea asigna virus 2A peptide (insect)6T2A
crePhage P1 Cre recombinase6cre
loxPphage P1 loxP6loxP
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal tail morphology(MP:0002111)

  • absent tail(MP:0003456)

  • curly tail(MP:0003051)

  • short tail(MP:0000592)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE2A [equine rhinitis A virus 2A peptide], human histone H2B cDNA,Discosoma sp. mCherry cDNA, T2A [Thosea asigna virus 2A peptide], Phage P1 Cre recombinase DNA, human Estrogen receptor cDNA, Phage P1 loxP site, mouse Noto genomic DNA
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0579K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mech Dev. 2009 126(10):791-803.) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. UCSD MTA
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data