Strain Data Sheet

RBRC05282

Strain Information

Image
BRC No.RBRC05282
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cd300lb<tm1Toki>/Rbrc
Former Common nameLMIR5 (Acc. No. CDB0539K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Toshio Kitamura, The Institute of Medical Science, The University of Tokyo.
Strain descriptionCd300lb, CD300 antigen like family member B knockout mice. Homozygous mutant mice are protected from renal IRI (ischemia reperfusion injury)
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
TIM1 is an endogenous ligand for LMIR5/CD300b: LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury.
Yamanishi Y, Kitaura J, Izawa K, Kaitani A, Komeno Y, Nakamura M, Yamazaki S, Enomoto Y, Oki T, Akiba H, Abe T, Komori T, Morikawa Y, Kiyonari H, Takai T, Okumura K, Kitamura T
J. Exp. Med., 207, 1501-1511 (2010). 20566714

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd300lbCD300 molecule like family member B11Cd300lbtargeted mutation 1, Toshio Kitamura
Frtyeast FRT (flippase recombination target) site11Frt
Frtyeast FRT (flippase recombination target) site11Frt
lacZbeta-galactosidase (E. coli)11
lox71mutant lox sequence11lox71
loxPphage P1 loxP11loxP
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • decreased susceptibility to kidney reperfusion injury(MP:0004042)

  • immune system phenotype(MP:0005387)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli (Neo, LacZ), phage P1 (lox71, loxP), rabbit beta-globin 3' end, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, yeast FRT (flipase recombination target) site, mouse Cd300lb genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Toshio Kitamura, The University of Tokyo, E-mail: kitamura@ims.u-tokyo.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0539K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2010 207(7):1501-11.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data