Strain Data Sheet

RBRC05281

Information1

Image
BRC No.RBRC05281
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ltbr<tm1Mmat>/Rbrc
Former Common nameLtbR (Acc. No. CDB0531K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Mitsuru Matsumoto, Institute for Enzyme Research, The University of Tokushima.
Strain descriptionLymphotoxin B receptor knockout mice. Homozygous mutant miceexhibit accumulation of lymphoid cell aggregates (B220+, CD4+, and CD8+ lymphocytes) in the liver and lung.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesJ. Immunol., 86, 5047-5057 (2011). 21441458

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ltbrlymphotoxin B receptor6Ltbr<tm1Mmat>targeted mutation 1, Mitsuru Matsumoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence6lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 lox71, E. coli lacZ, polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK3’end, phage P1 loxP site, rabbit beta-globin 3’end, mouse LtbR genomic DNA
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitsuru Matsumoto, The University of Tokushima, E-mail: mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0531K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Immunol. 2011 186(9):5047-57.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Oct 2013
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data