Strain Data Sheet

RBRC05281

Strain Information

Image
BRC No.RBRC05281
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ltbr<tm1Mmat>/Rbrc
Former Common nameLtbR (Acc. No. CDB0531K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Mitsuru Matsumoto, Institute for Enzyme Research, The University of Tokushima.
Strain descriptionLymphotoxin B receptor knockout mice. Homozygous mutant miceexhibit accumulation of lymphoid cell aggregates (B220+, CD4+, and CD8+ lymphocytes) in the liver and lung.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma.
Mouri Y, Yano M, Shinzawa M, Shimo Y, Hirota F, Nishikawa Y, Nii T, Kiyonari H, Abe T, Uehara H, Izumi K, Tamada K, Chen L, Penninger J M, Inoue J, Akiyama T, Matsumoto M
J. Immunol., 86, 5047-5057 (2011). 21441458

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site6Frt
Ltbrlymphotoxin B receptor6Ltbrtargeted mutation 1, Mitsuru Matsumoto
lacZbeta-galactosidase (E. coli)6
lox71mutant lox sequence6lox71
loxPphage P1 loxP6loxP
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal lymphocyte morphology(MP:0002619)

  • abnormal spleen morphology(MP:0000689)

  • abnormal thymus epithelium morphology(MP:0009544)

  • abnormal thymus medulla morphology(MP:0002375)

  • absent follicular dendritic cells(MP:0008201)
    • more 3 phenotypes
  • absent mesenteric lymph nodes(MP:0008465)

  • absent spleen germinal center(MP:0008474)

  • small thymus medulla(MP:0013588)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 lox71, E. coli lacZ, polyA, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK3’end, phage P1 loxP site, rabbit beta-globin 3’end, mouse LtbR genomic DNA
    Research application
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Mitsuru Matsumoto, The University of Tokushima, E-mail: mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0531K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Immunol. 2011 186(9):5047-57.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Oct 2013

    BRC mice in Publications

    No Data