Strain Data Sheet

RBRC05280

Strain Information

Image
BRC No.RBRC05280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namenrd1 (Acc. No.CDB0466K)
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Eiichirou Nishi, Graduate School of Medicine, Kyoto University.
Strain descriptionNrd1, nardilysin, N-arginine dibasic convertase, NRD convertase 1 knockout mice (Nrd1 (lacZ)). Homozygous mutant mice mostly die within 48 hours after birth. Surviving mice show small brain and thin cerebral cortex, cortical thinning, hypomyelination, reduced grip strength, impaired coordination and impaired spatial working memory.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
Nardilysin regulates axonal maturation and myelination in the central and peripheral nervous system.
Ohno M, Hiraoka Y, Matsuoka T, Tomimoto H, Takao K, Miyakawa T, Oshima N, Kiyonari H, Kimura T, Kita T, Nishi E
Nat. Neurosci., 12, 1506-1513 (2009). 19935654

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site4Frt
Nrd1nardilysin, N-arginine dibasic convertase, NRD convertase 14Nrd1targeted mutation 1, Eiichiro Nishi
lacZbeta-galactosidase (E. coli)4
loxPphage P1 loxP4loxP
neoneomycin resistance gene (E. coli)4

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal axon morphology(MP:0005404)

  • abnormal cerebral cortex morphology(MP:0000788)

  • abnormal myelin sheath morphology(MP:0003871)

  • abnormal myelination(MP:0000920)

  • abnormal sciatic nerve morphology(MP:0002651)
    • more 10 phenotypes
  • abnormal spatial working memory(MP:0008428)

  • decreased birth weight(MP:0009674)

  • decreased body size(MP:0001265)

  • decreased brain weight(MP:0002175)

  • decreased grip strength(MP:0010053)

  • enlarged lateral ventricles(MP:0008535)

  • impaired coordination(MP:0001405)

  • limb grasping(MP:0001513)

  • postnatal lethality, incomplete penetrance(MP:0011086)

  • thin cerebral cortex(MP:0006254)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli neo, yeast FRT (flipase recombination target) site, phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, E. coli lacZ, Rabbit beta globin pA signal, mouse nrd1 genomic DNA
    Research application
    Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Eiichirou Nishi, E-mail: nishi@kuhp.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0466K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat. Neurosci., 2009 12(12):1506-13.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data