RBRC05280

Information1

Image
BRC No.RBRC05280
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namenrd1 (Acc. No.CDB0466K)
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Eiichirou Nishi, Graduate School of Medicine, Kyoto University.
Strain descriptionNrd1, nardilysin, N-arginine dibasic convertase, NRD convertase 1 knockout mice (Nrd1 (lacZ)). Homozygous mutant mice mostly die within 48 hours after birth. Surviving mice show small brain and thin cerebral cortex, cortical thinning, hypomyelination, reduced grip strength, impaired coordination and impaired spatial working memory.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesNat. Neurosci., 12, 1506-1513 (2009). 19935654

Health Report

Examination Date / Room / Rack2013/06/17Room:3-CRack:B
2013/04/15Room:3-CRack:B
2013/02/18Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Nrd1nardilysin, N-arginine dibasic convertase, NRD convertase 14Nrd1<tm1Eiin>targeted mutation 1, Eiichiro Nishi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)4

Information2

Donor DNAE. coli neo, yeast FRT (flipase recombination target) site, phage P1 loxP, mouse phosphoglycerate kinase promoter (PGK promoter), mouse PGK3’end, E. coli lacZ, Rabbit beta globin pA signal, mouse nrd1 genomic DNA
Research application
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Eiichirou Nishi, E-mail: nishi@kuhp.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0466K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Nat. Neurosci., 2009 12(12):1506-13.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data