Strain Data Sheet

RBRC05275

Information1

Image
BRC No.RBRC05275
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pkdcc<tm1.1Mask>/Rbrc
Former Common nameVlk (Acc. No. CDB0434K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shin-Ichi Nishikawa, RIKEN Center for Developmental Biology.
Strain descriptionPkdcc, protein kinase domain containing, cytoplasmic knockout mice (24247-venus-LNL). Homozygous mice die soon after birth due to respiratory failure.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
ReferencesDevelopment, 136, 2069-2079 (2009). 19465597

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pkdccprotein kinase domain containing, cytoplasmic;17Pkdcc<tm1.1Mask>targeted mutation 1.1, Masaki Kinoshita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)17Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAattB1,Jellyfish venus (GFP) cDNA, phage P1 loxP site, attB2, mouse Pkdcc genomic DNA
Research applicationCre/loxP system
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0434K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Development. 2009 136(12):2069-79.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data