Strain Data Sheet

RBRC05269

Strain Information

Image
BRC No.RBRC05269
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Mir9-2<tm1Sia>/Rbrc
Former Common namemicroRNA9-2 (Acc. No. CDB0072K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Shinichi Aizawa, RIKEN Center for Developmental Biology.
Strain descriptionmicroRNA9-2 knockout mice. Homozygous mutant males die within 1 month after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6N]
References
MicroRNA-9 regulates neurogenesis in mouse telencephalon by targeting multiple transcription factors.
Shibata M, Nakao H, Kiyonari H, Abe T, Aizawa S
J. Neurosci., 31, 3407-3422 (2011). 21368052

Health Report

Examination Date / Room / Rack2023/03/13Room:3-5Rack:C
2022/12/12Room:3-5Rack:C

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal13
Mir9-2microRNA 9-213Mir9-2targeted mutation 1, Shinichi Aizawa
loxPphage P1 loxP13loxP
loxPphage P1 loxP13loxP
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • nervous system phenotype(MP:0003631)

  • premature death(MP:0002083)
    • Detailed phenotype data

    Ordering Information

    Donor DNABacteriophage-P1 loxP sites, Mouse (PGK promoter, PGK 3' end), E. coli Neo, simian virus 40 (SV40) poly(A) signal, mouse Mir9-2 genomic DNA
    Research applicationCre/loxP system
    Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0072K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Neurosci. 2011 31(9):3407-22.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
    DepositorRIKEN CDB (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data