Strain Data Sheet

RBRC05262

Strain Information

Image
BRC No.RBRC05262
TypeSpontaneous Mutation
SpeciesMus musculus
Strain name129.DW-Tpst2<grt>/Hkv
Former Common name129-grt
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki and Takashi Agui, Graduate School of Veterinary Medicine, Hokkaido University in 2010. Tpst2<grt> allele derived from DW/J-Tpst2<grt> was backcrossed to 129<+Ter>/SvJcl for 14 generations.
Strain descriptionTpst2<grt> 129 congenic strain. These mice show no hypothyroidism.
Colony maintenanceHomozygote x Heterozygote [or Crossing to 129<+Ter>/SvJcl]
References
A mutation in Tpst2 encoding tyrosylprotein sulfotransferase causes dwarfism associated with hypothyroidism.
Sasaki N, Hosoda Y, Nagata A, Ding M, Cheng J M, Miyamoto T, Okano S, Asano A, Miyoshi I, Agui T
Mol. Endocrinol., 21, 1713-1721 (2007). 17456791

Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background.
Hosoda Y, Sasaki N, Agui T
Biomed. Res. 31, 207-211 (2010). 20622471

Female infertility in grt mice is caused by thyroid hormone deficiency, not by insufficient TPST2 activity in the reproductive organs.
Hosoda Y, Sasaki N, Agui T
J. Vet. Med. Sci., 70, 1043-1049 (2008). 18981659

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tpst2protein-tyrosine sulfotransferase 25Tpst2protein-tyrosine sulfotransferase 2; growth retarded

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal adenohypophysis morphology(MP:0004163)

  • abnormal growth hormone level(MP:0003968)

  • abnormal postnatal growth/weight/body size(MP:0002089)

  • abnormal seminiferous tubule morphology(MP:0002216)

  • azoospermia(MP:0005159)
    • more 6 phenotypes
  • decreased activity of thyroid gland(MP:0003503)

  • decreased circulating thyroxine level(MP:0005478)

  • decreased testis weight(MP:0004852)

  • enlarged adenohypophysis(MP:0008366)

  • postnatal growth retardation(MP:0001732)

  • small seminiferous tubules(MP:0001153)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
    DepositorTakashi Agui (Hokkaido University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data