Strain Data Sheet

RBRC05251

Strain Information

Image
BRC No.RBRC05251
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(Pou2f3-nCre-ires-Venus)M2Imt
Former Common nameB6-Pou2f3-cre/Venus Tg#M2
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Ichiro Matsumoto, Monell Chemical Senses Center. The transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain descriptionThese transgenic mice express cre and Venus genes under the control of the Pou2f3 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Iresinternal ribosomal entry site (EMCV)UN
VenusYellow Fluorescent Protein (Aequorea victoria)UNVenus
crePhage P1 Cre recombinaseUNcremouse Pou2f3 promoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Pou2f3 genomic DNA, SV40 large T antigen Nuclear Localization Signal, Phage P1 Cre recombinase DNA, internal ribosomal entry site (EMCV), jellyfish Venus cDNA, PolyA signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must notify the content of the research to the DEPOSITOR and obtain a prior written consent on use of it from the DEPOSITOR. (Ichiro Matsumoto: ichiro.matsumoto1@gmail.com). The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
    Nat. Biotechnol., 20, 87-90 (2002).
    DepositorIchiro Matsumoto (Monell Chemical Senses Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data