Strain Data Sheet

RBRC05250

Strain Information

Image
BRC No.RBRC05250
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Pou2f3-Ascl1-ires-nEGFP)2Abek
Former Common nameB6-Pou2f3-Ascl1/GFP Tg#2
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ichiro Matsumoto (Graduate School of Agricultural and Life Sciences, The University of Tokyo / Monell Chemical Senses Center) and Keiko Abe (Graduate School of Agricultural and Life Sciences, The University of Tokyo). The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThese transgenic mice express Ascl1 and nGFP genes under the control of the Pou2f3 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
PolyA signalUN
Ascl1achaete-scute complex homolog-like 1 (Drosophila)UNAscl1
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
IRESinternal ribosomal entry site (EMCV)UN
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPou2f3 genomic DNA, Ascl1 cDNA, ires, large T antigen Nuclear Localization Signal, GFP cDNA, PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENIT of BIOLOGICAL RESOURCE must notify the content of the research to the DEPOSITOR and obtain a prior written consent on use of it from the DEPOSITOR. (Ichiro Matsumoto: ichiro.matsumoto1@gmail.com). The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the B|OLOGICAL RESOURCE.
    DepositorIchiro Matsumoto (Monell Chemical Senses Center)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data