RBRC05249

Information1

Image
BRC No.RBRC05249
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(Pou2f3-Ascl1-ires-nEGFP)1Abek
Former Common nameB6-Pou2f3-Ascl1/GFP Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Ichiro Matsumoto (Graduate School of Agricultural and Life Sciences, The University of Tokyo / Monell Chemical Senses Center) and Keiko Abe (Graduate School of Agricultural and Life Sciences, The University of Tokyo). The transgene was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThese transgenic mice express Ascl1 and nGFP genes under the control of the Pou2f3 promoter.
Colony maintenanceCarrier x Noncarrier [C57BL/6JJcl]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Ascl1achaete-scute complex homolog-like 1 (Drosophila)UNAscl1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele namePromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Information2

Donor DNAPou2f3 genomic DNA, Ascl1 cDNA, ires, large T antigen Nuclear Localization Signal, GFP cDNA, PolyA signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENIT of BIOLOGICAL RESOURCE must notify the content of the research to the DEPOSITOR and obtain a prior written consent on use of it from the DEPOSITOR. (Ichiro Matsumoto: ichiro.matsumoto1@gmail.com). The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the B|OLOGICAL RESOURCE.
DepositorIchiro Matsumoto (Monell Chemical Senses Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data