RBRC05243

Information1

Image
BRC No.RBRC05243
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pitx2<tm2.1Hmd>
Former Common nameB6-Pitx2c/lacZ KI
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hidetaka Shiratori and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2010. G4 ES cells derived from (129S6/SvEvTac x C57BL/6NCr)F1 were used to generate the mutant mice. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice.
Strain descriptionPitx2c knockout mice (lacZ reporter). Details unknown.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesDev. Biol., 395(2):331-41 (2014). 25224222

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Pitx2paired-like homeodomain transcription factor 23Pitx2<tm2.1Hmd>targeted mutation 2.1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site3Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)3

Information2

Donor DNAE. coli beta-galactosidase, yeast FRT (flippase recombination target) site, mouse Pitx2c genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorHiroshi Hamada (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data