Strain Data Sheet

RBRC05243

Strain Information

Image
BRC No.RBRC05243
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129B6-Pitx2<tm2.1Hmd>
Former Common nameB6-Pitx2c/lacZ KI
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Hidetaka Shiratori and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2010. G4 ES cells derived from (129S6/SvEvTac x C57BL/6NCr)F1 were used to generate the mutant mice. A frt flanked neo cassette was removed by crossing with CAG-FLPe mice.
Strain descriptionPitx2c knockout mice (lacZ reporter). Details unknown.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
References
Self-regulated left-right asymmetric expression of Pitx2c in the developing mouse limb.
Shiratori H, Yashiro K, Iwai N, Oki S, Minegishi K, Ikawa Y, Kanata K, Hamada H
Dev. Biol., 395(2):331-41 (2014). 25224222

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site3Frt
  • anterior segment dysgenesis 4(MedGEN)

  • Axenfeld-Rieger syndrome type 1(DisGeNET, MedGEN)

  • ring dermoid of cornea(MedGEN)
    • Pitx2paired-like homeodomain transcription factor 23Pitx2targeted mutation 2.1, Hiroshi Hamada
  • anterior segment dysgenesis 4(MedGEN)

  • Axenfeld-Rieger syndrome type 1(DisGeNET, MedGEN)

  • ring dermoid of cornea(MedGEN)
    • lacZbeta-galactosidase (E. coli)3
  • anterior segment dysgenesis 4(MedGEN)

  • Axenfeld-Rieger syndrome type 1(DisGeNET, MedGEN)

  • ring dermoid of cornea(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal aorta morphology(MP:0000272)

  • abnormal aortic arch morphology(MP:0004113)

  • abnormal atrioventricular cushion morphology(MP:0000297)

  • abnormal azygos vein morphology(MP:0011569)

  • abnormal cardiac outflow tract development(MP:0006126)
    • more 17 phenotypes
  • abnormal duodenum morphology(MP:0003271)

  • abnormal hepatic portal vein morphology(MP:0010668)

  • abnormal left-right axis patterning(MP:0001706)

  • abnormal pancreas morphology(MP:0001944)

  • abnormal sixth pharyngeal arch artery morphology(MP:0006355)

  • abnormal vena cava morphology(MP:0006062)

  • atrial septal defect(MP:0010403)

  • atrioventricular septal defect(MP:0010412)

  • common atrioventricular valve(MP:0010607)

  • dextrocardia(MP:0000644)

  • double outlet right ventricle(MP:0000284)

  • failure of atrioventricular cushion closure(MP:0000299)

  • right atrial isomerism(MP:0006061)

  • right pulmonary isomerism(MP:0000531)

  • small spleen(MP:0000692)

  • transposition of great arteries(MP:0004110)

  • ventricular septal defect(MP:0010402)
    • Detailed phenotype data

    Ordering Information

    Donor DNAE. coli beta-galactosidase, yeast FRT (flippase recombination target) site, mouse Pitx2c genomic DNA
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Biol., 395(2):331-41 (2014).
    DepositorHiroshi Hamada (RIKEN CDB)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data