RBRC05237

Information1

Image
BRC No.RBRC05237
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Col19a1<tm1Rmz>
Former Common name129-Col19a1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Hideaki Sumiyoshi (Oita Medical University), Francesco Ramirez (Weill College of Medical of Cornell University) and Hidekatsu Yoshioka (Oita Medical University).
Strain descriptionColl9a1 knockout mice. IRES-lacZ and PGK-neo cassette replaced exon 4 of Col19a1. Homozygous mutant mice show eating disorder by impaired sphincter relaxation in the lower esophagus, and more than 90% (in 129), 30-50% (in C57BL/6) of the mice die within 3 weeks after birth. Esophagus achalasia is also observed with aging.
Colony maintenanceHeterozygote x Wild-type
ReferencesJ. Cell Biol., 166, 591-600 (2004). 15302855

Health Report

Examination Date / Room / Rack2020/02/03Room:3-5Rack:D
2019/12/02Room:3-5Rack:D
2019/10/16Room:3-5Rack:D
2019/08/05Room:3-5Rack:D
2019/06/03Room:3-5Rack:D
2019/04/01Room:3-5Rack:D
2019/02/04Room:3-5Rack:D
2018/12/03Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Col19a1collagen, type XIX, alpha 11Col19a1<tm1Rmz>

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAEMCV internal ribosomal entry site, E. coli beta-galactosidase, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Col19a1 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Reproductive Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHidekatsu Yoshioka (Oita University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data