Strain Data Sheet

RBRC05237

Strain Information

Image
BRC No.RBRC05237
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129-Col19a1<tm1Rmz>
Former Common name129-Col19a1 KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Hideaki Sumiyoshi (Oita Medical University), Francesco Ramirez (Weill College of Medical of Cornell University) and Hidekatsu Yoshioka (Oita Medical University).
Strain descriptionColl9a1 knockout mice. IRES-lacZ and PGK-neo cassette replaced exon 4 of Col19a1. Homozygous mutant mice show eating disorder by impaired sphincter relaxation in the lower esophagus, and more than 90% (in 129), 30-50% (in C57BL/6) of the mice die within 3 weeks after birth. Esophagus achalasia is also observed with aging.
Colony maintenanceHeterozygote x Wild-type
References
Esophageal muscle physiology and morphogenesis require assembly of a collagen XIX-rich basement membrane zone.
Sumiyoshi H, Mor N, Lee S Y, Doty S, Henderson S, Tanaka S, Yoshioka H, Rattan S, Ramirez F
J. Cell Biol., 166, 591-600 (2004). 15302855

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Col19a1collagen, type XIX, alpha 11Col19a1targeted mutation 1,
IRESinternal ribosomal entry site (EMCV)1
lacZbeta-galactosidase (E. coli)1
neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal esophageal smooth muscle morphology(MP:0003159)

  • abnormal esophagus development(MP:0003160)

  • abnormal esophagus morphology(MP:0000467)

  • dilated esophagus(MP:0004548)

  • dysphagia(MP:0003158)
    • more 2 phenotypes
  • postnatal lethality, incomplete penetrance(MP:0011086)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAEMCV internal ribosomal entry site, E. coli beta-galactosidase, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Col19a1 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Reproductive Biology Research
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHidekatsu Yoshioka (Oita University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data