Strain Data Sheet

RBRC05223

Strain Information

Image
BRC No.RBRC05223
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR;B6D2-Tg(CAG-Luc,Hsd17b3/EGFP)1Tysn
Former Common nameICR-CAG-Luc,Hsd17b3/EGFP Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tsuyoshi Nakanishi, graduate school of pharmaceutical sciences, Osaka University in 2007. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. ICR mixed background.
Strain descriptionThese transgenic mice express a 17betaHSD type3 and GFP fusion protein by cre-mediated recombination.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
  • 46,XY disorder of sex development due to 17-beta-hydroxysteroid dehydrogenase 3 deficiency(MedGEN)
    • Hsd17b3hydroxysteroid (17-beta) dehydrogenase 3UNHsd17b3
  • 46,XY disorder of sex development due to 17-beta-hydroxysteroid dehydrogenase 3 deficiency(MedGEN)
    • loxPphage P1 loxPUNloxP
  • 46,XY disorder of sex development due to 17-beta-hydroxysteroid dehydrogenase 3 deficiency(MedGEN)
    • loxPphage P1 loxPUNloxP
  • 46,XY disorder of sex development due to 17-beta-hydroxysteroid dehydrogenase 3 deficiency(MedGEN)
    • lucluciferase (firefly)UNlucCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
  • 46,XY disorder of sex development due to 17-beta-hydroxysteroid dehydrogenase 3 deficiency(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), P1 Phage loxP sites, Mouse 17beta-hydroxysteroid dehydrogenase type3, Firefly Luciferase cDNA, Jellyfish Enhanced green fluorescent protein (EGFP) cDNA, SV40 Poly A signal
    Research applicationCre/loxP system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorTsuyoshi Nakanishi (Gifu Pharmaceutical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. CAGGS MTA (English)
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data