Strain Data Sheet

RBRC05203

Strain Information

Image
BRC No.RBRC05203
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(TH-ALK*F1174L)5Homy
Former Common nameB6-Th-ALK/F1174L Tg#5L
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine and Junko Takita, The University of Tokyo Hospital. A F1174L mutant hALK gene under the TH promoter was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain descriptionHuman ALK (anaplastic lymphoma kinase) gene having F1174L mutation transgenic mice. F1174L mutation was identified in a patient with an inflammatory myofibroblastic tumor harboring a RANBP2-ALK translocation.
Colony maintenance
References
Oncogenic mutations of ALK kinase in neuroblastoma.
Yuyan Chen, Junko Takita, Young Lim Choi, Motohiro Kato, Miki Ohira, Masashi Sanada, Lili Wang, Manabu Soda, Akira Kikuchi, Takashi Igarashi, Akira Nakagawara, Yasuhide Hayashi, Hiroyuki Mano, Seishi Ogawa
Nature, 455, 971-974 (2008). 18923524

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ALK human ALK cDNA (F1174L mutant)UNALK human tyrosine hydroxylase promoter

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal sympathetic ganglion morphology (MP:0001008)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman tyrosine hydroxylase promoter, human ALK cDNA (F1174L mutant), rabbit beta globin intron, Herpes simplex virus HSV terminator
    Research applicationCancer Research
    Mouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data