Strain Data Sheet

RBRC05202

Strain Information

Image
BRC No.RBRC05202
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Mbtd1<tm2Homy>
Former Common nameHemp cKO B6-Mbtd1 floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 6 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 7 times.
Strain descriptionMbtd1 (Hemp) gene floxed mice. Exon 6 of Mbtd1 gene is flanked by loxP sites. Conditional Mbtd1 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frtyeast FRT (flippase recombination target) site11Frt
Mbtd1mbt domain containing 111Mbtd1 targeted mutation 2, Hiroaki Honda
loxPphage P1 loxP11loxP
loxPphage P1 loxP11loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Mbtd1 genomic DNA, Phage P1 loxP, yeast FRT (flipase recombination target) site
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data