Strain Data Sheet

RBRC05201

Strain Information

Image
BRC No.RBRC05201
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Mbtd1<tm1Homy>
Former Common nameHemp KO B6-Mbtd1 KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Mbtd1 mutant mice were generated by replacing the mbt-coding exons with IRES-GFP and floxed neo resistant gene. The neo resistant gene was removed by microinjecting a Cre expression vector into fertilized eggs. The mutant mice were backcrossed with C57BL/6N for at least 10 times.
Strain descriptionMbtd1 (Hemp) gene knockout mice. Exon 6, 7 and 8 (mbt-coding exons) were replaced with an IRES-GFP. Western blot analysis showed absence of Mbtb1 protein. Homozygous null mutant mice show skeletal abnormalities and are neonatal lethal.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References
Hemp, an mbt domain-containing protein, plays essential roles in hematopoietic stem cell function and skeletal formation.
Hiroaki Honda, Keiyo Takubo, Hideaki Oda, Kenjiro Kosaki, Tatsuya Tazaki, Norimasa Yamasaki, Kazuko Miyazaki, Kateri A Moore, Zen-ichiro Honda, Toshio Suda, Ihor R Lemischka
Proc. Natl. Acad. Sci. USA, 108, 2468-2473 (2011). 21252303

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)11
IRES internal ribosomal entry site (EMCV)11
Mbtd1
MGI:2143977		mbt domain containing 111Mbtd1<tm1Homy>
MGI:4940055		targeted mutation 1, Hiroaki Honda
loxP phage P1 loxP11loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cervical atlas morphology (MP:0004607)

  • abnormal cervical axis morphology (MP:0004608)

  • abnormal cervical vertebrae morphology (MP:0003048)

  • abnormal costovertebral joint morphology (MP:0008149)

  • abnormal interparietal bone morphology (MP:0000077)
    • more 16 phenotypes
  • abnormal lambdoid suture morphology (MP:0003841)

  • abnormal liver development (MP:0000596)

  • abnormal neurocranium morphology (MP:0000074)

  • abnormal occipital bone morphology (MP:0005269)

  • abnormal skeleton morphology (MP:0005508)

  • abnormal spine curvature (MP:0004174)

  • abnormal thoracic cage morphology (MP:0004624)

  • abnormal vertebral column morphology (MP:0004703)

  • cervical vertebral fusion (MP:0004620)

  • decreased hematopoietic stem cell number (MP:0004810)

  • flat occipital bone (MP:0030289)

  • liver hypoplasia (MP:0000600)

  • neonatal lethality, incomplete penetrance (MP:0011088)

  • occipital bone hypoplasia (MP:0030286)

  • rib bifurcation (MP:0000153)

  • rib fusion (MP:0000154)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Mbtd1 genomic DNA, jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)

    BRC mice in Publications

    No Data