RBRC05201

Information1

Image
BRC No.RBRC05201
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Mbtd1<tm1Homy>
Former Common nameHemp KO B6-Mbtd1 KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Mbtd1 mutant mice were generated by replacing the mbt-coding exons with IRES-GFP and floxed neo resistant gene. The neo resistant gene was removed by microinjecting a Cre expression vector into fertilized eggs. The mutant mice were backcrossed with C57BL/6N for at least 10 times.
Strain descriptionMbtd1 (Hemp) gene knockout mice. Exon 6, 7 and 8 (mbt-coding exons) were replaced with an IRES-GFP. Western blot analysis showed absence of Mbtb1 protein. Homozygous null mutant mice show skeletal abnormalities and are neonatal lethal.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
ReferencesProc. Natl. Acad. Sci. USA, 108, 2468-2473 (2011). 21252303

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mbtd1mbt domain containing 111Mbtd1<tm1Homy>targeted mutation 1, Hiroaki Honda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GFPGreen Fluorescent Protein (Jellyfish)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
IRESinternal ribosomal entry site (EMCV)11

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP11loxP

Information2

Donor DNAmouse Mbtd1 genomic DNA, jellyfish GFP cDNA, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 loxP
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP (Japanese)

BRC mice in Publications

No Data