RBRC05200

Information1

Image
BRC No.RBRC05200
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Kdm2b<tm1.1Homy>
Former Common nameFbxl10 KO B6-Kdm2b KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 8 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 8. Exon 8 was removed by crossing with Cre mice (B6. B6C3-Tg (CAG-Cre) CZ-M02Osb). The mice were backcrossed with C57BL/6N for at least 9 times.
Strain descriptionKdm2b (Fbxl10) gene knockout mice. Exon 8 was removed by cre-mediated recombination.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Kdm2blysine (K)-specific demethylase 2B5Kdm2b <tm1.1Homy>targeted mutation 1.1, Hiroaki Honda

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site5Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Information2

Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Kdm2b genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHiroaki Honda (Tokyo Women's Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Lab HP (Japanese)

BRC mice in Publications

No Data