Strain Data Sheet

RBRC05200

Strain Information

Image
BRC No.RBRC05200
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Kdm2b<tm1.1Homy>
Former Common nameFbxl10 KO B6-Kdm2b KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 8 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 8. Exon 8 was removed by crossing with Cre mice (B6. B6C3-Tg (CAG-Cre) CZ-M02Osb). The mice were backcrossed with C57BL/6N for at least 9 times.
Strain descriptionKdm2b (Fbxl10) gene knockout mice. Exon 8 was removed by cre-mediated recombination.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Frt yeast FRT (flippase recombination target) site5Frt
Kdm2b
MGI:1354737		lysine (K)-specific demethylase 2B5Kdm2b <tm1.1Homy> targeted mutation 1.1, Hiroaki Honda
loxP phage P1 loxP5loxP
neo neomycin resistance gene (E. coli)5 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP site, yeast FRT (flipase recombination target) sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Kdm2b genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)

    BRC mice in Publications

    No Data