Strain Data Sheet

RBRC05199

Strain Information

Image
BRC No.RBRC05199
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Kdm2b<tm1Homy>
Former Common nameFbxl10 cKO B6-Kdm2b floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 8 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 8. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 8 times.
Strain descriptionKdm2b (Fbxl10) gene floxed mice. Exon 8 of Kdm2b gene is flanked by loxP sites. Conditonal Kdm2b deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Kdm2b
MGI:1354737		lysine (K)-specific demethylase 2B5Kdm2b<tm1Homy> targeted mutation 1, Hiroaki Honda
loxP phage P1 loxP5loxP
loxP phage P1 loxP5loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Kdm2b genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Nishimura H, Ikawa Y, Kajikawa E, Shimizu-Mizuno N, Hiver S, Tabata-Okamoto N, Mori M, Kitajima T, Hayashi T, Yoshimura M, Umeda M, Nikaido I, Kurokawa M, Watanabe T, Hamada H.
    Maternal epigenetic factors in embryonic and postnatal development.
    Genes Cells 28(6) 422-432(2023) 36906847