Strain Data Sheet

RBRC05198

Strain Information

Image
BRC No.RBRC05198
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Ciz1<tm1Homy>
Former Common nameCiz1 cKO B6-Ciz1 floxed
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2008. A loxP was inserted upstream of exon 5 and an FRT flanked neo cassette with a 3’ loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 6 times.
Strain descriptionCiz1 gene floxed mice. Exon 5 of Ciz1 gene is flanked by loxP sites. Conditonal Ciz1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NCrlCrlj]
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Ciz1
MGI:1920234		CDKN1A interacting zinc finger protein 12Ciz1<tm1Homy> targeted mutation 1, Hiroaki Honda
Frt yeast FRT (flippase recombination target) site2Frt
loxP phage P1 loxP2loxP
loxP phage P1 loxP2loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal tumor incidence (MP:0002019)

  • increased cellular sensitivity to hydroxyurea (MP:0008409)

  • increased leukemia incidence (MP:0002026)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Ciz1 genomic DNA, Phage P1 loxP, yeast FRT (flipase recombination target) site
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorHiroaki Honda (Tokyo Women's Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    Xiao J, Khan MM, Vemula S, Tian J, LeDoux MS.
    Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice.
    FEBS Lett 592(18) 3101-3110(2018) 30098009