Strain Data Sheet

RBRC05169

Strain Information

Image
BRC No.RBRC05169
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Th-FLPo)5Imayo
Former Common namepTH-FLPo line5
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. Transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionFLPo recombinase is expressed under the TH promoter.
Colony maintenance
References
A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.
Imayoshi I, Hirano K, Sakamoto M, Miyoshi G, Imura T, Kitano S, Miyachi H, Kageyama R
Neurosci. Res., 73, 85-91 (2012). 22343123

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLPoyeast flippase recombinase, a codon improved version of the flpe site specific recombinaseUNFLPorat tyrosine hydroxylase (TH) promoter
  • TH-deficient dopa-responsive dystonia(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat tyrosine hydroxylase (TH) promoter, yeast FLPo (synthetic) cDNA, poly A, rat tyrosine hydroxylase (TH) genomic DNA
    Research applicationFLP/frt system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neursci. Res., 73, 85-91 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data