Strain Data Sheet

RBRC05168

Strain Information

Image
BRC No.RBRC05168
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(Th-FLPo)4Imayo
Former Common namepTH-FLPo line4
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. Transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain descriptionFLPo recombinase is expressed under the TH promoter.
Colony maintenance
References
A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.
Imayoshi I, Hirano K, Sakamoto M, Miyoshi G, Imura T, Kitano S, Miyachi H, Kageyama R
Neurosci. Res., 73, 85-91 (2012). 22343123

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLPoyeast flippase recombinase, a codon improved version of the flpe site specific recombinaseUNFLPorat tyrosine hydroxylase (TH) promoter
  • TH-deficient dopa-responsive dystonia(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNArat tyrosine hydroxylase (TH) promoter, yeast FLPo (synthetic) cDNA, poly A, rat tyrosine hydroxylase (TH) genomic DNA
    Research applicationFLP/frt system
    Neurobiology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neursci. Res., 73, 85-91 (2012).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data