Strain Data Sheet

RBRC05167

Information1

Image
BRC No.RBRC05167
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Slc17a7<tm1.2(IRES-tTAad)Imayo>
Former Common nameVGLUT1-IRES2-tTA-Ad
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. loxP-Stop-loxP and loxP-sTOP-loxP cassettes were removed by crossing with pCAG-FLPe and TNAP-Cre mice (C57BL/6).
Strain descriptiontTA-Ad is expressed in VGLUT1-expressing cells. IRES2-tTA-Ad sequence is knocked into 3’UTR region of VGLUT1 gene.
Colony maintenance
ReferencesThe Journal of Neuroscience, 34, 5788-5799 (2014). 24760839

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc17a7solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 77Slc17a7<tm1.2(IRES-tTAad)Imayo>targeted mutation 1.2, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAadtTA-Advanced (synthetic)7tTAad

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal7

Information2

Donor DNABacteriophage P1 loxP site, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), tTA-Advanced (synthetic), SV40 early polyA signal, Yeast FRT (flippase recombination target) site, mouse Slc17a7 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Itaru Imayoshi HP

BRC mice in Publications

No Data