RBRC05165

Information1

Image
BRC No.RBRC05165
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo>
Former Common nameVGLUT1-LoxP-Stop-LoxP-IRES2-EGFP-TeNT
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain development
Strain descriptionEGFP-TeNT is expressed after Cre-recombination in VGLUT1-expressing cells. LoxP-Stop-LoxP-IRES2-EGFP-TeNT sequence is knocked into 3’UTR region of VGLUT1 gene.
Colony maintenance
ReferencesThe Journal of Neuroscience, 34, 5788-5799 (2014). 24760839

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Slc17a7solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 77Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo>targeted mutation 1.1, Itaru Imayoshi

Information2

Donor DNABacteriophage P1 loxP sites, SV40 early polyA signal, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, Yeast FRT (flippase recombination target) site
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP

BRC mice in Publications

No Data