RBRC05158

Information1

Image
BRC No.RBRC05158
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-ChR2/mCherry)Imayo>
Former Common nameR26-CAG-FRT-ChR2(C128A)-mCherry
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain development
Strain descriptionChR2 (C128A) -mCherry is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, ChR2 (C128A) -mCherry is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.
Colony maintenance
ReferencesNeurosci. Res., 75(1):53-58 (2013). 22465523

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.2(CAG-ChR2/mCherry)Imayo>targeted mutation 1.2, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
ChR2 (C128A)Chlamydomonas Channelrhodopsin-2 mutant C128A6ChR2 (C128A)CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
GHgrowth hormone polyadenylation signal6GH

Gene symbolGene nameChr.Allele symbolAllele namePromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)6puro

Gene symbolGene nameChr.Allele symbolAllele namePromoter
HSV thymidine kinase polyA signal6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 early polyA signal6

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 late polyA signal6

Information2

Donor DNAS. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP site, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Chlamydomonas Channelrhodopsin-2 mutant C128A, Discosoma sp. mCherry cDNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):53-58 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Dr. Karl Deisseroth, MTA
Itaru Imayoshi HP

BRC mice in Publications

No Data