Strain Data Sheet
RBRC05158
Information1 | |
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| Image | |
| BRC No. | RBRC05158 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-ChR2/mCherry)Imayo> |
| Former Common name | R26-CAG-FRT-ChR2(C128A)-mCherry |
| H-2 Haplotype | |
| ES Cell line | TC1 [129S6/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. loxP-Stop-loxP cassette was removed by crossing with TNAP-Cre mice (C57BL/6). |
| Strain description | ChR2(C128A)-mCherry is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, ChR2(C128A)-mCherry is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. |
| Colony maintenance | |
| References | Neurosci. Res., 75(1):53-58 (2013). 22465523 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.2(CAG-ChR2/mCherry)Imayo>targeted mutation 1.2, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ChR2 (C128A)Chlamydomonas Channelrhodopsin-2 mutant C128A6ChR2 (C128A)CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHgrowth hormone polyadenylation signal6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin resistance gene (Streptomyces alboniger)6puro Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 late polyA signal6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HSV thymidine kinase polyA signal6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 early polyA signal6 |
Information2 | |
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| Donor DNA | S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP site, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Chlamydomonas Channelrhodopsin-2 mutant C128A, Discosoma sp. mCherry cDNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):53-58 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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