Strain Data Sheet
RBRC05156
Strain Information | |
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| Image | |
| BRC No. | RBRC05156 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S6-Gt(ROSA)26Sor<tm1(CAG-ChR2/mCherry)Imayo> |
| Former Common name | R26-CAG-LF-ChR2(C128A)-mCherry |
| H-2 Haplotype | |
| ES Cell line | TC1 [129S6/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. Crossed to C57BL/6. |
| Strain description | ChR2(C128A)-mCherry is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, ChR2(C128A)-mCherry is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. |
| Colony maintenance | |
| References | Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0. Imayoshi I, Tabuchi S, Hirano K, Sakamoto M, Kitano S, Miyachi H, Yamanaka A, Kageyama R Neurosci. Res., 75(1):53-58 (2013). 22465523 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| SV40 early polyA signal | 6 | ||||||
| HSV thymidine kinase polyA signal | 6 | ||||||
| SV40 late polyA signal | 6 | ||||||
| ChR2 | channelrhodopsin-2 (Chlamydomonas reinhardtii) | 6 | ChR2 | CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) | |||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| Frt | yeast FRT (flippase recombination target) site | 6 | Frt | ||||
| GH | growth hormone polyadenylation signal | 6 | GH | ||||
| Gt(ROSA)26Sor | gene trap ROSA 26, Philippe Soriano | 6 | Gt(ROSA)26Sor | targeted mutation 1, Itaru Imayoshi | |||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | 6 | WPRE | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| loxP | phage P1 loxP | 6 | loxP | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | 6 | mCherry | ||||
| puro | puromycin acyltransferase (Streptomyces alboniger) | 6 | puro | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) sites, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), Chlamydomonas Channelrhodopsin-2 mutant C128A cDNA, Discosoma sp. mCherry cDNA, mouse Gt(ROSA)26Sor genomic region |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):53-58 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR. |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |