RBRC05154

Information1

Image
BRC No.RBRC05154
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-EGFP/TeNT)Imayo>
Former Common nameR26-CAG-LoxP-EGFP-TeNT
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain development
Strain descriptionEGFP-TeNTpest is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. EGFP-TeNTpest is expressed under CAG promoter.
Colony maintenance
ReferencesThe Journal of Neuroscience, 34, 5788-5799 (2014). 24760839

Health Report

Examination Date / Room / Rack2018/03/26Room:4-ARack:F
2018/01/22Room:4-ARack:F
2017/11/27Room:4-ARack:F
2017/10/30Room:4-ARack:F
2017/09/25Room:4-ARack:F
2017/08/21Room:4-ARack:F
2017/08/21Room:4-ARack:F
2017/07/24Room:4-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)6EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Odc1mouse ornithine decarboxylase (PEST sequence) cDNA6Odc1

Gene symbolGene nameChr.Allele symbolAllele namePromoter
TeNTtetanus neurotoxin light chain (Clostridium tetani)6TeNT

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
poly ApolyA6poly A

Gene symbolGene nameChr.Allele symbolAllele namePromoter
puropuromycin resistance gene (Streptomyces alboniger)6puro

Information2

Donor DNAsplice acceptor, S. alboniger puromycin resistance gene, Bovine growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) site, Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, mouse PEST sequence, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP

BRC mice in Publications

No Data