Strain Data Sheet
RBRC05153
Information1 | |
|---|---|
| Image | |
| BRC No. | RBRC05153 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S6-Gt(ROSA)26Sor<tm1(CAG-EGFP/TeNT)Imayo> |
| Former Common name | R26-CAG-LF-EGFP-TeNT |
| H-2 Haplotype | |
| ES Cell line | TC1 [129S6/SvEvTac] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. Crossed to C57BL/6. |
| Strain description | EGFP-TeNTpest is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. EGFP-TeNTpest is expressed under CAG promoter. |
| Colony maintenance | |
| References | The Journal of Neuroscience, 34, 5788-5799 (2014). 24760839 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-EGFP/TeNT)Imayo>targeted mutation 1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)6EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Odc1mouse ornithine decarboxylase (PEST sequence) cDNA6Odc1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TeNTtetanus neurotoxin light chain (Clostridium tetani)6TeNT Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly ApolyA6poly A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin resistance gene (Streptomyces alboniger)6puro |
Information2 | |
|---|---|
| Donor DNA | splice acceptor, S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) sites, Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, mouse PEST sequence, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
| Depositor | Itaru Imayoshi (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
|
BRC mice in Publications |
|---|
| No Data |