Strain Data Sheet

RBRC05152

Strain Information

Image
BRC No.RBRC05152
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>
Former Common nameR26-CAG-FRT-eNpHR2-EYFP
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. loxP-Stop-loxP cassette was removed by crossing with TNAP-Cre mice (C57BL/6).
Strain descriptioneNpHR2-EYFP is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.
Colony maintenance
References
Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0.
Imayoshi I, Tabuchi S, Hirano K, Sakamoto M, Kitano S, Miyachi H, Yamanaka A, Kageyama R
Neurosci. Res., 75(1):53-58 (2013). 22465523

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)6EYFP
Frtyeast FRT (flippase recombination target) site6Frt
Frtyeast FRT (flippase recombination target) site6Frt
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1.2, Itaru Imayoshi
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE
eNpHR2Natronomonas enhanced halorhodopsin 26eNpHR2
loxPphage P1 loxP6loxP
poly ApolyA6poly A
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNASA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 loxP site, SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Yeast FRT (flippase recombination target) sites, Natronomonas enhanced halorhodopsin 2 (eNpHR2), Jellyfish EYFP cDNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), mouse Gt(ROSA)26Sor genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):53-58 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Dr. Karl Deisseroth, MTA
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data