Strain Data Sheet

RBRC05151

Information1

Image
BRC No.RBRC05151
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo>
Former Common nameR26-CAG-LoxP-eNpHR2-EYFP
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6).
Strain descriptioneNpHR2-EYFP is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.
Colony maintenance
ReferencesNeurosci. Res., 75(1):53-58 (2013). 22465523

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EYFPEnhanced Yellow Fluorescent Protein (Aequorea victoria)6EYFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
eNpHR2Natronomonas enhanced halorhodopsin 26eNpHR2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin resistance gene (Streptomyces alboniger)6puro

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tpASV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal6tpA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Information2

Donor DNASA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 loxP sites, SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Yeast FRT (flippase recombination target) site, Natronomonas enhanced halorhodopsin 2 (eNpHR2), Jellyfish EYFP cDNA, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 75(1):53-58 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Dr. Karl Deisseroth, MTA
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Itaru Imayoshi HP

BRC mice in Publications

No Data