Strain Data Sheet

RBRC05149

Strain Information

Image
BRC No.RBRC05149
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.3(CAG-mTFP1)Imayo>
Former Common nameR26-CAG-mTFP1
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. loxP-Stop-loxP and loxP-sTOP-loxP cassettes were removed by crossing with pCAG-FLPe and TNAP-Cre mice (C57BL/6).
Strain descriptionHAtag-attached mTFP1 fluorescent protein is expressed throughout body. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.
Colony maintenance
References
A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination.
Imayoshi I, Hirano K, Sakamoto M, Miyoshi G, Imura T, Kitano S, Miyachi H, Kageyama R
Neurosci. Res., 73, 85-91 (2012). 22343123

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal6
Frtyeast FRT (flippase recombination target) site6Frt
GHgrowth hormone polyadenylation signal6GH
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sortargeted mutation 1.3, Itaru Imayoshi
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA
SASA (splice acceptor sequence)6SA
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE
loxPphage P1 loxP6loxP
mTFP1monomeric Teal Fluorescent Protein 1 (Clavularia sp.)6mTFP1
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • no abnormal phenotype detected(MP:0002169)

  • obsolete no phenotypic analysis(MP:0003012)
    • Detailed phenotype data

    Ordering Information

    Donor DNASA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Bacteriophage P1 loxP site, Yeast FRT (flippase recombination target) site, HA (hemagglutinin) tag sequence, Clavularia sp. mTFP1, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), SV40 polyA signal
    Research applicationFLP/frt system
    Fluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 73, 85-91 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
    DepositorItaru Imayoshi (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. CAGGS MTA (English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Itaru Imayoshi HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data