Strain Data Sheet

RBRC05147

Information1

Image
BRC No.RBRC05147
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>
Former Common nameR26-CAG-LoxP-mTFP1
H-2 Haplotype
ES Cell lineTC1 [129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6).
Strain descriptionHAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region.
Colony maintenance
ReferencesNeurosci. Res., 73, 85-91 (2012). 22343123

Health Report

Examination Date / Room / Rack2023/08/21Room:4-ARack:J
2023/05/22Room:4-ARack:J
2023/02/20Room:4-ARack:J
2022/11/21Room:4-ARack:J
2022/08/22Room:4-ARack:J
2022/05/23Room:4-ARack:J
2022/03/07Room:4-ARack:J
2021/12/06Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>targeted mutation 1.1, Itaru Imayoshi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHgrowth hormone polyadenylation signal6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASA (splice acceptor sequence)6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mTFP1monomeric Teal Fluorescent Protein 1 (Clavularia sp.)6mTFP1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tkHSV thymidine kinase polyA signal6tk

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 late polyA signal6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
STOP cassette (SV40pA-TKpA-SV40pA)6

Information2

Donor DNASA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), HA (hemagglutinin) tag sequence, Clavularia sp. mTFP1, STOP cassette
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 73, 85-91 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
DepositorItaru Imayoshi (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Itaru Imayoshi HP

BRC mice in Publications

Kase Y, Otsu K, Shimazaki T, Okano H.
Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling.
Stem Cell Reports 12(6) 1313-1328(2019) 31080114