Information1 | |
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Image | |
BRC No. | RBRC05147 |
Type | Targeted Mutation![]() |
Species | Mus musculus |
Strain name | B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo> |
Former Common name | R26-CAG-LoxP-mTFP1 |
H-2 Haplotype | |
ES Cell line | TC1 [129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6). |
Strain description | HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3’UTR region. |
Colony maintenance | |
References | Neurosci. Res., 73, 85-91 (2012). 22343123 |
Health Report | |
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Examination Date / Room / Rack | 2023/08/21Room:4-ARack:J 2023/05/22Room:4-ARack:J 2023/02/20Room:4-ARack:J 2022/11/21Room:4-ARack:J 2022/08/22Room:4-ARack:J 2022/05/23Room:4-ARack:J 2022/03/07Room:4-ARack:J 2021/12/06Room:4-ARack:J |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>targeted mutation 1.1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHgrowth hormone polyadenylation signal6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HAhuman influenza virus HA (hemagglutinin) tag sequence6HA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASA (splice acceptor sequence)6SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mTFP1monomeric Teal Fluorescent Protein 1 (Clavularia sp.)6mTFP1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)6puro Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tkHSV thymidine kinase polyA signal6tk Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 late polyA signal6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter STOP cassette (SV40pA-TKpA-SV40pA)6 |
Information2 | |
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Donor DNA | SA (splice acceptor sequence), S. alboniger puromycin resistance gene, growth hormone polyadenylation signal, CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV-IE enhancer), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal, Bacteriophage P1 loxP sites, Yeast FRT (flippase recombination target) site, Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), HA (hemagglutinin) tag sequence, Clavularia sp. mTFP1, STOP cassette |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurosci. Res., 73, 85-91 (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
Depositor | Itaru Imayoshi (Kyoto University) |
Strain Status | ![]() ![]() ![]() |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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Kase Y, Otsu K, Shimazaki T, Okano H. Involvement of p38 in Age-Related Decline in Adult Neurogenesis via Modulation of Wnt Signaling. Stem Cell Reports 12(6) 1313-1328(2019) 31080114 |