RBRC05145

Information1

Image
BRC No.RBRC05145
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Prdm9<tm1Ymat>
Former Common nameB6-Meisetz KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Hayashi and Yasuhisa Matsui, Osaka Medical Center for Maternal and Child Health in 1995. A targeting vector containing the NLS-lacZ and a loxP-flanked PGFneo cassette was inserted into the initiation codon of exon 2, all of exons 3 to 6, and part of exon 7. The mutant mice were backcrossed to C57BL/6JJcl.
Strain descriptionMeisetz (Prdm9) knockout mice. Homozygous mutant mice show meiotic arrest and both female and male are infertile.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesNature, 438, 374-378 (2005). 16292313

Health Report

Examination Date / Room / Rack2015/11/30Room:4-ARack:J
2015/09/28Room:4-ARack:J
2015/07/27Room:4-ARack:J
2015/05/25Room:4-ARack:J
2015/04/08Room:4-ARack:J
2015/04/06Room:4-ARack:J
2015/03/23Room:4-ARack:J
2015/01/19Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Prdm9PR domain containing 917Prdm9<tm1Ymat>targeted mutation 1, Yasuhisa Matsui

Gene symbolGene nameChr.Allele symbolAllele namePromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 PolyA signal17

Information2

Donor DNASimian virus 40 Large T antigen nuclear localization signal (NLS), E. coli Beta galactosidase gene, SV40 PolyA signal, Phage P1 loxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Prdm9 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature. 438, 374-378 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYasuhisa Matsui (Tohoku University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data