Strain Data Sheet

RBRC05144

Strain Information

Image
BRC No.RBRC05144
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Ifitm3-EGFP)3.0GYmat
Former Common nameB6-Ifitm3-GFP Tg#3.0G
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Satomi Tanaka and Yasuhisa Matsui, Osaka Medical Center for Maternal and Child Health in 2004. Transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6J.
Strain descriptionTransgenic mice having a 3.0-kb upstream flanking region of exon 1 of Ifitm3 fused in frame with the GFP. Tg mice express GFP in primordial germ cells (PGCs).
Colony maintenanceCarrier x Noncarrier [C57BL/6JJmsSlc]
References
Regulation of expression of mouse interferon-induced transmembrane protein like gene-3, Ifitm3 (mil-1, fragilis), in germ cells.
Tanaka S S, Nagamatsu G, Tokitake Y, Kasa M, Tam P P, Matsui Y
Dev. Dyn., 230, 651-659 (2004). 15254899

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP
GHGrowth hormone polyA (Bovine)UNGH

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • normal phenotype(MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse mil1 (fragilis) gene genomic DNA, jellyfish EGFP gene cDNA, bovine growth hormone PolyA signal
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Dyn., 230, 651-659(2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYasuhisa Matsui (Tohoku University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data