RBRC05143

Information1

Image
BRC No.RBRC05143
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S2-Fad158<tm1Maim>/7Maim
Former Common nameB6-Fad158 KO#7
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain development
Strain descriptionFad158 (Lrrc8c) knockout mice. Homozygous mutnat mice do not show body weight gain and insulin resistance by a high-fat diet. Developed by Graduate School of Pharmaceutical Sciences, Nagoya City University and Genome Information Research Center, Osaka University in 2006. A neomycin selection cassette was inserted into the exon 4 of the Fad158 gene. C57BL/6J background.
Colony maintenanceHeterozygote x Wild-type
ReferencesJ. Biol. Chem., 279, 34840-34848 (2004). 15184384
Biol. Pharm. Bull., 34, 1257-1263 (2011). 21804215

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Lrrc8cleucine rich repeat containing 8 family, member C5Lrrc8c<tm1Maim>targeted mutation 1, Masayoshi Imagawa

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse Pgk1 promoter, E. coli neomycin resistance gene, mouse Pgk1 polyA, mouse Fad158 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Pharm Bull. 2011 34(8):1257-63. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr.Makoto Nishizuka (nishizuka@hirosaki-u.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayoshi Imagawa (Nagoya City University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data