Strain Data Sheet

RBRC05119

Information1

Image
BRC No.RBRC05119
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC;B6CB-Tnf<tm1Sek>
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kenji Sekikawa, STAFF-Institute in 1997. The mice were backcrossed to BALB/c for at least 12 times by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo.
Strain descriptionTNF alpha knockout mice. Homozygous mutant mice show increased susceptibility to infection.
Colony maintenance
ReferencesLab. Invest., 77, 647-658 (1997). 9426403

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tnftumor necrosis factor17Tnf<tm1Sek>targeted mutation 1, Kenji Sekikawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse tnf genomic DNA, Neomycin resistance gene (E. coli), Phosphoglycerate kinase (PGK) promoter (mouse)
Research applicationImmunology and Inflammation Research
covid19_onset
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Lab. Invest., 77, 647-658 (1997).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested.
DepositorYoichiro Iwakura (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data