Strain Data Sheet

RBRC05118

Strain Information

Image
BRC No.RBRC05118
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Tnf<tm1Sek>
Former Common name
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kenji Sekikawa, STAFF-Institute in 1997. The mice were backcrossed to C57BL/6 for at least 12 times by Yoichiro Iwakura, The Institute of Medical Science, The University of Tokyo.
Strain descriptionTNF alpha knockout mice. Homozygous mutant mice show increased susceptibility to infection.
Colony maintenance
References
Failure of germinal center formation and impairment of response to endotoxin in tumor necrosis factor alpha-deficient mice.
Taniguchi T, Takata M, Ikeda A, Momotani E, Sekikawa K
Lab. Invest., 77, 647-658 (1997). 9426403

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:JSentinel mouse program
2024/05/27Room:4-BRack:JSentinel mouse program
2024/02/26Room:4-BRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tnf
MGI:104798		tumor necrosis factor17Tnf<tm1Sek>
MGI:2653694		targeted mutation 1, Kenji Sekikawa
  • inherited susceptibility to asthma(MedGEN)

  • malaria, susceptibility to(MedGEN)

  • migraine with or without aura, susceptibility to, 1(MedGEN)
    • neo neomycin resistance gene (E. coli)17 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Peyer's patch germinal center morphology (MP:0002391)

  • abnormal cytokine secretion (MP:0003009)

  • abnormal female reproductive system physiology (MP:0003699)

  • abnormal liver physiology (MP:0000609)

  • abnormal macrophage physiology (MP:0002451)
    • more 18 phenotypes
  • abnormal mesenteric lymph node morphology (MP:0005232)

  • abnormal serotonin level (MP:0005322)

  • abnormal vagina weight (MP:0008978)

  • absent lymph node germinal center (MP:0008523)

  • absent spleen germinal center (MP:0008474)

  • decreased circulating interferon-gamma level (MP:0008578)

  • decreased circulating interleukin-1 alpha level (MP:0008639)

  • decreased circulating interleukin-1 beta level (MP:0008642)

  • decreased circulating interleukin-6 level (MP:0008597)

  • decreased coping response (MP:0003064)

  • decreased locomotor activity (MP:0001402)

  • decreased susceptibility to endotoxin shock (MP:0008734)

  • decreased vertical activity (MP:0002757)

  • immune system phenotype (MP:0005387)

  • increased anxiety-related response (MP:0001363)

  • increased grooming behavior (MP:0001441)

  • increased serotonin level (MP:0010069)

  • prolonged estrus (MP:0009017)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Tnf genomic DNA
    Research applicationImmunology and Inflammation Research
    covid19_onset
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Lab. Invest., 77, 647-658 (1997). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorYoichiro Iwakura (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data