Strain Data Sheet

RBRC05073

Information1

Image
BRC No.RBRC05073
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(CAG-flag-H2A)
Former Common nameB6-Flag/H2A Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nashun Buhe, Masashi Yukawa and Fugaku Aoki, Graduate School of Frontier Sciences, The University of Tokyo in 2009.
Strain descriptionFlag-H2A (Hist3h2a) transgenic mice.
Colony maintenanceCarrier x Noncarrier
ReferencesGenomics, 80, 487-98 (2002). 12408966
Development, 137, 3785-3794 (2010). 20943707

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hist3h2ahistone cluster 3, H2aUNHist3h2aCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Flag-tag cDNA, Mouse Histone H2A (Hist3h2a) gene cDNA
Research application
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorFugaku Aoki (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Oct 2011
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data