RBRC05065

Information1

Image
BRC No.RBRC05065
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Sox1<tm1(cre)Take>
Former Common nameB6-Sox1/cre KI
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentCre recombinase was inserted into the ATG codon start site if Sox1 by in-frame. PGK-neo cassette was removed by Flp recombinase expression.
Strain descriptionSox1 cre knock-in mice. knocked into the Sox1 gene. No obvious defect at the heterozygous state which is used for the conditional KO or cell tracing purposes.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
ReferencesCell, 129, 1377-1388 (2007). 17604725

Health Report

Examination Date / Room / Rack2020/06/23Room:3-CRack:B
2020/06/01Room:3-CRack:B
2020/02/17Room:3-CRack:B
2019/12/16Room:3-CRack:B
2019/10/21Room:3-CRack:B
2019/08/26Room:3-CRack:B
2019/06/17Room:3-CRack:B
2019/04/15Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Sox1SRY-box containing gene 18Sox1<tm1(cre)Take>targeted mutation 1, Takumi Era

Gene symbolGene nameChr.Allele symbolAllele namePromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
crePhage P1 Cre recombinase8cre

Information2

Donor DNAPhage P1 cre recombinase, yeast FRT (flippase recombination target) site, mouse Sox1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and Conditions(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0525K, and Web Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Cell, 129, 1377-1388 (2007).)
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (within 1 month)
Additional Info.Genotyping protocol -PCR-

BRC mice in Publications

No Data