RBRC04952

Information1

Image
BRC No.RBRC04952
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Fstl4<tm1Ngo>
Former Common nameB6-Mahya-2 KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tatsuhiko Kadowaki, Graduate School of Bioagricultural Sciences, Nagoya University in 2006.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJmsSlc]
ReferencesDev. Genes Evol., 215, 564-574 (2005). 16193321

Health Report

Examination Date / Room / Rack2013/02/18Room:3-DRack:E
2012/12/10Room:3-DRack:E
2012/10/29Room:3-DRack:E
2012/10/23Room:3-DRack:E
2012/10/15Room:3-DRack:E
2012/10/15Room:3-DRack:E
2012/08/27Room:3-DRack:E
2012/06/18Room:3-DRack:E

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fstl4follistatin-like 411Fstl4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusGreen Fluorescent Protein, F46L (Jellyfish)11VenusVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAE. coli neo, P1 Phage loxP, Mouse PGK promoter, PGK 3'end, Jelly fish Venus
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Genes Evol., 215, 564-574 (2005).
DepositorTatsuhiko Kadowaki (Nagoya University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).

BRC mice in Publications

Maekawa Y, Onishi A, Matsushita K, Koide N, Mandai M, Suzuma K, Kitaoka T, Kuwahara A, Ozone C, Nakano T, Eiraku M, Takahashi M.
Optimized Culture System to Induce Neurite Outgrowth From Retinal Ganglion Cells in Three-Dimensional Retinal Aggregates Differentiated From Mouse and Human Embryonic Stem Cells.
Curr. Eye Res. 41(4) 558-68(2015) 25880804